Two high density lipoprotein fractions (HDL₁ and HDL₂) were isolated from the hemolymphatic plasma of the spider Latrodectus mirabilis (Holmberg 1876). For each, the hydrated density, the electrophoresis mobility of the apoproteins, and the lipid classes composition were determined. The HDL₁ fraction carried 80% of the total plasma lipids, which were predominantly composed of phospholipids, free fatty acids, and triacylglycerols. The apoprotein composition of this fraction showed two main bands of 90 and 103 kDa. The HDL₂ fraction was composed primarily of phospholipids, free fatty acids and cholesterol. This fraction contained hemocyanin as the principal apoprotein. When the HDL₂ fraction was separated into three subfractions, all of them contained hemocyanin, with the main subfraction containing the hexameric form of the respiratory pigment. With regard to triacylglycerol transport, lipid and apoprotein compositions and hemocyanin role in the lipid transport, these lipoproteins (HDL₁, HDL₂) show similarities and differences when compared to the two spider species already studied.